Applications
Automating colony PCR screening
Screening hundreds of colonies is high-throughput, low-precision work. Where a colony PCR workflow can relax and where it still cannot cut corners.
Applications
Liquid handling in context: assays, sample prep, and workflows where getting the volume right decides the result.
- Applications
Automating digital PCR (dPCR) setup
Digital PCR counts molecules by partitioning a reaction thousands of ways. Why that makes the setup pipetting unusually unforgiving, and how to meet the bar.
- Applications
Automating reverse transcription and RT-qPCR setup
RNA is fragile and the RT step sets the ceiling on everything downstream. The cold-chain, RNase, and one-step versus two-step choices that automation has to respect.
- Applications
Multiplex PCR: pooling and balancing primer sets on a liquid handler
A multiplex works only if no primer pair crowds out the rest. The many small oligo transfers and ratio balancing that building a pool actually requires.
- Applications
Building qPCR standard curves by automated serial dilution
A standard curve is only as good as its dilutions. How log-scale error propagates, and the pipetting discipline that keeps a curve linear and efficient.
- Applications
Normalizing nucleic acid input before PCR
Equal template mass per well is what makes results comparable. The per-well dilution math and the small-volume transfers that make normalization real.
- Applications
Preparing and distributing PCR master mix at scale
The batch math, dead volume, and viscosity handling that keep the first well and the last well of a plate carrying the same reaction.
- Applications
Pipetting glycerol from 50 to 100 percent: a viscosity ladder
Glycerol is not one liquid. Its viscosity climbs steeply with concentration, so the settings that work at 50 percent are wrong at 80 and useless at 100.
- Applications
Handling blood, plasma, and serum: films, clots, and unreliable surfaces
Biological fluids clot, foam from protein, and coat the tip in a film that fools level detection. What a class for whole blood or serum has to account for.
- Applications
Sample prep for LC-MS/MS: precise transfers where small volumes decide the result
Mass spec quantitation is only as good as the standards and dilutions feeding it. Here is how to keep low-volume transfers accurate for LC-MS/MS.
- Applications
Automating ELISA: the liquid-handling settings that protect reproducibility
ELISA lives or dies on even, gentle, well-timed liquid handling. Here are the dispense, wash, and reagent-addition settings that keep plates consistent.
- Applications
Automating PCR and qPCR setup: master mix, small volumes, no cross-contamination
Setting up PCR plates by hand is tedious and error prone. The liquid-class settings that protect ratio fidelity at a few microliters and keep amplicon out.
- Applications
Concentration normalization: turning a plate of readings into equal concentrations
Normalization takes a plate of unequal concentrations to a common target. Variable volumes, one class, and a minimum-transfer floor that decides feasibility.
- Applications
Compound management in DMSO: hygroscopic, viscous, and unforgiving
DMSO is the backbone solvent of compound libraries and one of the hardest liquids to pipette well. Viscosity, water uptake, and freeze-thaw all move the target.
- Applications
Magnetic-bead cleanup: aspirating around the pellet without losing it
SPRI and magnetic-bead cleanups live or die on supernatant removal. The class settings that clear liquid off an engaged pellet without carrying beads away.
- Applications
NGS library prep on a liquid handler: small volumes, tight ratios, unforgiving error budgets
Library prep stacks dozens of small, ratio-sensitive transfers. Here is how to automate it without letting pipetting error compound into failed libraries.